Anti Complement factor B (chicken)
mouse monoclonal antibody
Cat.No. HYB 022-03
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Complement factor B plays an important role in the alternative complement activation pathway by complexing with C3b to create the active C3 convertase. The alternative activation pathway was the first to evolve, thus the components involved are very similar in physological different species.
Complement factor B isolated from chicken plasma (1)
HYB 022-03 is specific for the Bb fragment of chicken factor B, both native and denatured.
Not determined
In Western blotting after SDS-PAGE, HYB 022-03 reacts with the three different phenotypes of factor B, either generating four fast running bands (phenotype F), four slow running bands (type S) or six bands (type S/F) (1). In ELISA, HYB 022-03 shows strong reaction with factor B (chicken) coated directly onto the microtiter well.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:15000 | |
| Immunoblotting | Yes | 1 | |
| Immunohistochemistry | Not determined |
1. Koch C (1986) A genetic polymorphism of the complement component factor B in chickens not linked to the major histocompatibility complex (MHC). Immunogenetics 23:364-367.
2. Kjalke M, Welinder KG, Koch C (1993) Structural analysis of chicken factor B-like protease and comparison with mammalian complement proteins factor B and C2. J Immunol 151:4147-4152.
3. Koch C (1986) The alternative complement pathway in chickens. Purification of factor B and production of a monospecific antibody against it. Acta Pathol Microbiol Immunol Scand C 94:253-259.
4. Koch C, Skjodt K, Laursen I (1985) A simple immunoblotting method after separation of proteins in agarose gel. J Immunol Methods 84:271-278.
Downloads
HYB 022-03.pdf (14.86 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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April 23 2008
